Chinese translation for "recombinant bacteria"
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- 重组细菌
Related Translations:
- Example Sentences:
| 1. | These indicated that the porcine il - 6 gene was correctly transcribed and translated in the recombinant bacteria . the fusion protein was recovered from polyacrylamid gel and used to immunize the rabbit , and the liter of rabbit anti - porcine il - 6 serum from immunized rabbit was 1 : 128 , 000
从制备性聚丙烯酰胺凝胶中回收融合蛋白，用mtt法测定重组蛋白刺激小鼠脾淋巴母细胞增殖反应活性，并以重组蛋白免疫家兔制备抗猪il - 6滴度为128 ， 000的高免兔血清。 | | 2. | The results were found that the specific 670bp dna bases of cgh was detected by rt - pcr in the recombinant bacteria and a new protein band was found in sds - page with molecular mass of about 50 kda which is consisted of a 23 . 6 kda protein deduced from the cgh gene sequence and gst ( 26 kda )
Rt - pcr分析确证重组质粒在大肠杆菌中能正确转录； sds - page分析证明诱导重组质粒表达了融合蛋白，分子量约为50kd ，与预期的26kd的gst带和23 . 6kd的草鱼生长激素基因编码蛋白质构成的融合蛋白大小一致。 | | 3. | The mrna and protein expression were assayed by rt - pcr and sds - page . the results were found that the specific 740bp dna bases of il - 6 was detected by rt - pcr in the recombinant bacteria and a new protein band was found in sds - page with molecular mass of about 49 kda which is consisted of a 23 kda protein deduced from the il - 6 gene sequence and gst ( 26 kda )
以iptg诱导融合蛋白表达，经rt - pcr检测发现740bp的特异性il - 6条带；通过sds - page分析，在49kd处出现gst ? pil6融合蛋白条带，其表达量占总细菌蛋白量的30 ，证明猪il - 6基因得到了正确转录和表达。 | | 4. | The expression conditions of e2 gene in p . pastoris were optimized , the results indicated that the peak obtained after 72 hours ; pattern of inhibition / induction could improve expression level ; the best ph value were between 7 . 5 and 8 . 0 and the optimized methanol - induced concentration was 2 % - 3 % the e2 genes of the prevalent strain ( guangxi yulin strain ) and c strain derived from rabbit spleen tissue were amplified and cloned into e . coli the expression vector pproex - htb respectively , the recombinant plasmids pproex - gxyl and pproex - c were obtained and then were transformed into the dh5a e . coli competent bacteria respectively , the recombinant bacteria could express the major antigen region of e2 gene , the expression yields amount to 35 % and 38 % repectively
猪瘟病毒ez基因的原核表达： pcr扩增出当前猪瘟流行野毒株，中国猪瘟兔化弱毒（ c株）兔脾组织毒ez基因的主要抗原区，将其克隆到原核大肠杆菌表达载体pproex htb中诱导表达，经sds page检测表明，重组质粒能表达ez基因主要区蛋白， westernblot检测表明，诱导表达蛋白与猪瘟阳性血清发生特异性反应，表达量为35和38 ，可用于基因工程诊断抗原。 |
- Similar Words：
- "recom redstone computer" Chinese translation, "recombed top" Chinese translation, "recombinagen" Chinese translation, "recombinagenic" Chinese translation, "recombinant" Chinese translation, "recombinant chromosome" Chinese translation, "recombinant clones" Chinese translation, "recombinant deficient mutant" Chinese translation, "recombinant deficient mutant of bacteria" Chinese translation, "recombinant deficient mutant of phage" Chinese translation
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